Reach-To-Grasp Movements: A Multimodal Techniques Study

The aim of the present study was to investigate the correlation between corticospinal activity, kinematics, and electromyography (EMG) associated with the execution of precision and whole-hand grasps (WHGs). To this end, motor-evoked potentials (MEPs) induced by transcranial magnetic stimulation (TMS), EMG, and 3-D motion capture data have been simultaneously recorded during the planning and the execution of prehensile actions toward either a small or a large object. Differences in the considered measures were expected to distinguish between the two types of grasping actions both in terms of action preparation and execution. The results indicate that the index finger (FDI) and the little finger (ADM) muscles showed different activation patterns during grasping execution, but only the FDI appeared to distinguish between the two types of actions during motor preparation. Kinematics analysis showed that precision grips differed from WHGs in terms of displayed fingers distance when shaping before object\u2019s contact, and in terms of timing and velocity patterns. Moreover, significant correlations suggest a relationship between the muscular activation and the temporal aspects concerned with the index finger\u2019s extension during whole-hand actions. Overall, the present data seem to suggest a crucial role played by index finger as an early \u201cmarker\u201d of differential motor preparation for different types of grasps and as a \u201cnavigator\u201d in guiding whole-hand prehensile actions. Aside from the novelty of the methodological approach characterizing the present study, the data provide new insights regarding the level of crosstalk among different levels concerned with the neuro-behavioral organization of reach-to-grasp movements

Look at Me: Early Gaze Engagement Enhances Corticospinal Excitability During Action Observation

Direct gaze is a powerful social cue able to capture the onlooker's attention. Beside gaze, head and limb movements as well can provide relevant sources of information for social interaction. This study investigated the joint role of direct gaze and hand gestures on onlookers corticospinal excitability (CE). In two experiments we manipulated the temporal and spatial aspects of observed gaze and hand behavior to assess their role in affecting motor preparation. To do this, transcranial magnetic stimulation (TMS) on the primary motor cortex (M1) coupled with electromyography (EMG) recording was used in two experiments. In the crucial manipulation, we showed to participants four video clips of an actor who initially displayed eye contact while starting a social request gesture, and then completed the action while directing his gaze toward a salient object for the interaction. This way, the observed gaze potentially expressed the intention to interact. Eye tracking data confirmed that gaze manipulation was effective in drawing observers' attention to the actor's hand gesture. In the attempt to reveal possible time-locked modulations, we tracked CE at the onset and offset of the request gesture. Neurophysiological results showed an early CE modulation when the actor was about to start the request gesture looking straight to the participants, compared to when his gaze was averted from the gesture. This effect was time-locked to the kinematics of the actor's arm movement. Overall, data from the two experiments seem to indicate that the joint contribution of direct gaze and precocious kinematic information, gained while a request gesture is on the verge of beginning, increases the subjective experience of involvement and allows observers to prepare for an appropriate social interaction. On the contrary, the separation of gaze cues and body kinematics can have adverse effects on social motor preparation. CE is highly susceptible to biological cues, such as averted gaze, which is able to automatically capture and divert observer's attention. This point to the existence of heuristics based on early action and gaze cues that would allow observers to interact appropriately

The Effect of Water Spray Systems on Thermal and Solar Performance of an ETFE Panel for Building Envelope

ETFE membranes are generally used in architecture for large roofing and façade systems, because of their transparency and lightness compared to glass alternatives. Multilayer ETFE panels are used to improve single membrane systems performances, reducing thermal losses, by the use of an air gap between two or more ETFE foils, generally serigraphed or surface treated to reduce solar gains. Surface temperatures and global solar radiation strongly affects mean radiant temperature (MRT), and comfort perceived by a user facing a transparent envelope as well as solar gains strongly influences primary energy use for cooling in summer conditions. In the following paper an alternative dynamic solar gains mitigation strategy is presented and applied to a double layer, non-cushions, ETFE panel for façades. We measured the effectiveness of a water spray system located in the air-gap between the parallel ETFE foils and used to reduce surface temperatures and solar access depending on different summer solar radiation values and outdoor/indoor air temperature conditions. Systems alternative with different in nozzle dimension, water spray geometry and water consumption were already tested to evaluate the best compromise between solar gains reduction and water use. The results are preliminary but we noticed that a reduction up to the 10% of the total solar gains could be achieved as well as a reduction of 10 °C of surface temperature. Comfort evaluation for a standard indoor space were already done

Mindreading in the balance : adults' mediolateral leaning and anticipatory looking foretell others' action preparation in a false-belief interactive task

Anticipatory looking on mindreading tasks can indicate our expectation of an agent's action. The challenge is that social situations are often more complex, involving instances where we need to track an agent's false belief to successfully identify the outcome to which an action is directed. If motor processes can guide how action goals are understood, it is conceivable— where that kind of goal ascription occurs in false-belief tasks— for motor representations to account for someone's belief-like state. Testing adults (N = 42) in a real-time interactive helping scenario, we discovered that participants' early mediolateral motor activity (leftwards– rightwards leaning on balance board) foreshadowed the agent's belief-based action preparation. These results suggest fast belief-tracking can modulate motor representations generated in the course of one's interaction with an agent. While adults' leaning, and anticipatory looking, revealed the contribution of fast false-belief tracking, participants did not correct the agent's mistake in their final helping action. These discoveries suggest that adults may not necessarily use another's belief during overt social interaction or find reflecting on another's belief as being normatively relevant to one's own choice of action. Our interactive task design offers a promising way to investigate how motor and mindreading processes may be variously integrated

Gaze and Body Cues Interplay during Interactive Requests

Although observing other\u2019s gaze and body movements provides a crucial source of information to successfully interact with other people, it remains unclear whether observers weigh differently these cues and whether the convergence of gaze and body\u2019s directions determines facilitation effects. Here we aim to shed more light on this issue by testing the reliance upon these cues from both a behavioral and a neurophysiological perspective in a social interactive context. In Experiment 1, we manipulated the convergence between the direction of an actor\u2019s upper limb movement and gaze direction while he attempts to socially interact with the participants observing the scene. We determined the direction of gaze as well as the duration of participants\u2019 ocular fixations during the observation of the scene. In Experiment 2, we measured and correlated the effect of the body/gaze manipulation on corticospinal excitability and on the readiness to interact\u2014a disposition to engage in social situations. Eye-tracking data revealed that participants fixated chiefly the actor\u2019s head when his hand and gaze directions were divergent. Possibly a strategy to disambiguate the scene. Whereas participants mainly fixated the actor\u2019s hand when he performed an interactive request toward the participants. From a neurophysiological point of view, the more participants felt involved in the interaction, the lower was motor preparation in the muscle potentially needed to fulfill the actor\u2019s request. We contend that social contexts are more likely to elicit motor preparation compared to non-social ones, and that muscular inhibition is a necessary mechanism in order to prevent unwanted overt reactions during action observation tasks

Experimental Assessment of the Reflection of Solar Radiation from Façades of Tall Buildings to the Pedestrian Level

Urban climates are highly influenced by the ability of built surfaces to reflect solar radiation, and the use of high-albedo materials has been widely investigated as an effective option to mitigate urban overheating. While diffusely solar reflective walls have attracted concerns in the architectural and thermal comfort community, the potential of concave and polished surfaces, such as glass and metal panels, to cause extreme glare and localized thermal stress has been underinvestigated. Furthermore, there is the need for a systematic comparison of the solar concentration at the pedestrian level in front of tall buildings. Herein, we show the findings of an experimental campaign measuring the magnitude of the sunlight reflected by scale models reproducing archetypical tall buildings. Three 1:100 scaled prototypes with different shapes (classic vertical façade, 10% tilted façade, curved concave façade) and different finishing materials (representative of extremes in reflectance properties of building materials) were assessed. A specular surface was assumed as representative of a glazed façade under high-incidence solar angles, while selected light-diffusing materials were considered sufficient proxies for plaster finishing. With a diffusely reflective façade, the incident radiation at the pedestrian level in front of the building did not increase by more than 30% for any geometry. However, with a specular reflective (i.e., mirror-like) flat façade, the incident radiation at the pedestrian level increased by more than 100% and even by more than 300% with curved solar-concentrating geometries. In addition, a tool for the preliminary evaluation of the solar reflectance risk potential of a generic complex building shape is developed and presented. Our findings demonstrate that the solar concentration risk due to mirror-like surfaces in the built environment should be a primary concern in design and urban microclimatology

Key role of MEK/ERK pathway in sustaining tumorigenicity and in vitro radioresistance of embryonal rhabdomyosarcoma stem-like cell population

The identification of signaling pathways that affect the cancer stem-like phenotype may provide insights into therapeutic targets for combating embryonal rhabdomyosarcoma. The aim of this study was to investigate the role of the MEK/ERK pathway in controlling the cancer stem-like phenotype using a model of rhabdospheres derived from the embryonal rhabdomyosarcoma cell line (RD)

Hypoxia sustains glioblastoma radioresistance through ERKs/DNA-PKcs/HIF-1α functional interplay

The molecular mechanisms by which glioblastoma multiforme (GBM) refracts and becomes resistant to radiotherapy treatment remains largely unknown. This radioresistance is partly due to the presence of hypoxic regions, which are frequently found in GBM tumors. We investigated the radiosensitizing effects of MEK/ERK inhibition on GBM cell lines under hypoxic conditions. Four human GBM cell lines, T98G, U87MG, U138MG and U251MG were treated with the MEK/ERK inhibitor U0126, the HIF-1α inhibitor FM19G11 or γ-irradiation either alone or in combination under hypoxic conditions. Immunoblot analysis of specific proteins was performed in order to define their anti‑oncogenic or radiosensitizing roles in the different experimental conditions. MEK/ERK inhibition by U0126 reverted the transformed phenotype and significantly enhanced the radiosensitivity of T98G, U87MG, U138MG cells but not of the U251MG cell line under hypoxic conditions. U0126 and ERK silencing by siRNA reduced the levels of DNA protein kinase catalytic subunit (DNA-PKcs), Ku70 and K80 proteins and clearly reduced HIF-1α activity and protein expression. Furthermore, DNA-PKcs siRNA-mediated silencing counteracted HIF-1α activity and downregulated protein expression suggesting that ERKs, DNA-PKcs and HIF-1α cooperate in radioprotection of GBM cells. Of note, HIF-1α inhibition under hypoxic conditions drastically radiosensitized all cell lines used. MEK/ERK signal transduction pathway, through the sustained expression of DNA-PKcs, positively regulates HIF-1α protein expression and activity, preserving GBM radioresistance in hypoxic condition

Mindreading by body: incorporating mediolateral balance and mouse-tracking measures to examine the motor basis of adults’ false-belief tracking

The role played by motor representations in tracking others’ belief-based actions remains unclear. In experiment 1, the dynamics of adults’ anticipatory mediolateral motor activity (leftwards–rightwards leaning on a balance board) as well as hand trajectories were measured as they attempted to help an agent who had a true or false belief about an object’s location. Participants’ leaning was influenced by the agent’s belief about the target’s location when the agent was free to act but not when she was motorically constrained. However, the hand trajectories participants produced to provide a response were not modulated by the other person’s beliefs. Therefore, we designed a simplified second experiment in which participants were instructed to click as fast as possible on the location of a target object. In experiment 2, mouse-movements deviated from an ideal direct path to the object location, with trajectories that were influenced by the location in which the agent falsely believed the object to be located. These experiments highlight that information about an agent’s false-belief can be mapped onto the motor system of a passive observer, and that there are situations in which the motor system plays an important role in accurate belief-tracking

A Case of Atypical Bartonellosis in a 4-Year-Old Immunocompetent Child

In most cases, infection due to Bartonella henselae causes a mild disease presenting with a regional lymphadenopathy frequently associated with a low-grade fever, headache, poor appetite and exhaustion that spontaneously resolves itself in a few weeks. As the infection is generally transmitted by cats through scratching or biting, the disease is named cat scratch disease (CSD). However, in 5-20% of cases, mainly in immunocompromised patients, systemic involvement can occur and CSD may result in major illness. This report describes a case of systemic CSD diagnosed in an immunocompetent 4-year-old child that can be used as an example of the problems that pediatricians must solve to reach a diagnosis of atypical CSD. Despite the child's lack of history suggesting any contact with cats and the absence of regional lymphadenopathy, the presence of a high fever, deterioration of their general condition, increased inflammatory biomarkers, hepatosplenic lesions (i.e., multiple abscesses), pericardial effusion with mild mitral valve regurgitation and a mild dilatation of the proximal and medial portion of the right coronary artery, seroconversion for B. henselae (IgG 1:256) supported the diagnosis of atypical CSD. Administration of oral azithromycin was initiated (10 mg/kg/die for 3 days) with a progressive normalization of clinical, laboratory and US hepatosplenic and cardiac findings. This case shows that the diagnosis of atypical CSD is challenging. The nonspecific, composite and variable clinical features of this disease require a careful evaluation in order to achieve a precise diagnosis and to avoid both a delayed diagnosis and therapy with a risk of negative evolution